Item# 1096: Recombinant HIV-1 Clades B, C Envelope gp41 and and HIV-2 Envelope gp36 Composite Protein (E.coli)
Concentration: See vial
Mass/vial: See vial
Volume/vial: see vial
Diluent: 10 mM Tris, pH8,0.002%SDS
Physical State: Frozen Liquid
Stability: 1 year at -200C, 3 years at -750C.
Applications:In-Vitro Diagnostics, Immunization,T-Cell Activation
Description: Truncated Recombinant HIV-1 clades B,C Envelope gp41 proteins and HIV-2 envelope gp36 protein expressed as a composite protein in the E.coli Expression System.
Purification: This protein is purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies specific for HIV-1 gp41 and HIV-2 gp36 and to HIV-1 and HIV-2 converted human serum polyclonal antibodies as determined by Elisa, Western Elisa and by lateral flow one-step HIV-1 and HIV-2 human diagnostics tests. Qualified for Lateral Flow HIV-1/2 Diagnostics.
Biological Activity: Not done
Application and instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.