HIV-1 ADA gp41

$435.00
SKU: 1089
Size: 100 ug

Product Specifictions

Item#1089: Recombinant HIV-1 ADA ( M-Tropic) Envelope Protein gp41 (E.coli).

 

Concentration: See vial 

 

Mass/vial: 0.1mg

 

Volume/vial: See Vial

 

Diluent: 10 mM Tris, pH 8.0, SDS (<0.01%)

 

Purity : >99%

 

Stabilizer: None

 

Preservative: None

 

Storage: -75°C

 

Physical State: Frozen Liquid

 

Stability: At least 24 months at -75oC.

 

Applications: In-Vitro Diagnostics, Immunization, T-Cell Activation

 

Description: Truncated Recombinant HIV-1 ADA M-Tropic envelope protein gp41 produced as a fusion protein in the E.coli Expression System. Truncated gp41 protein mol.wt: 25kD.

 

Purification: This protein is purified by affinity chromatography and by preparative electrophoresis to >99% purity as determined by SDS-PAGE, reduced.

 

Specificity: This protein binds to murine anti-gp41 mAbs and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Elisa.

 

Biological activity: T-cell activation assays pending.

 

Application and instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 0.1 – 0.5mg/ml.