Item#1089: Recombinant HIV-1 ADA ( M-Tropic) Envelope Protein gp41 (E.coli).
Concentration: See vial
Volume/vial: See Vial
Diluent: 10 mM Tris, pH 8.0, SDS (<0.01%)
Purity : >99%
Physical State: Frozen Liquid
Stability: At least 24 months at -75oC.
Applications: In-Vitro Diagnostics, Immunization, T-Cell Activation
Description: Truncated Recombinant HIV-1 ADA M-Tropic envelope protein gp41 produced as a fusion protein in the E.coli Expression System. Truncated gp41 protein mol.wt: 25kD.
Purification: This protein is purified by affinity chromatography and by preparative electrophoresis to >99% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine anti-gp41 mAbs and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Elisa.
Biological activity: T-cell activation assays pending.
Application and instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 0.1 – 0.5mg/ml.
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