Item# 1084: Recombinant HIV-1 ADA Envelope Glycoprotein gp160 ( Baculovirus)
Concentration: See vial
Mass/vial: See vial
Physical State: Frozen Liquid
Stability: 24 months at -75°C.
Application: CD Binding, Cell Activation, In-Vitro Diagnostics
Description: Gene cloned from M cell-tropic HIV-1 ADA strain and the truncated recombinant envelope gp160 glycoprotein produced in the Baculovirus Expression System.
Purification: This protein is purified by immuno-affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: Not determined.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions.