HCV rNS5 Protein (E.coli)

SKU: 4006
Size: 100 ug


Product # 4006:  Recombinant HCV NS5b Protein



SPECIFICATIONS                                                                APPLICATIONS

  Concentration:             see vial                                                   In-Vitro HCV Diagnostics

  Mass/vial:                   see vial                                                    Western Analysis

  Volume/vial:                see vial                                                 

  Diluent:                      PBS, 10% Glycerol                                                                                                         Purity               >95%                                         

  Stabilizer:                   None                                                      

  Preservative:                None                                                    

  Storage:                      -75o C

  Stability:                     At least 24 months at -75o C


DESCRIPTION:                       HCV NS5b  DNA  sequence expressed as a fusion protein in the E. coli expression system. MW: approx. 78kD



PURIFICATION: HCV NS5b protein was purified by solvent extraction and preparative gel electrophoresis                                                                                  to >95% purity as determined by SDS-PAGE, reduced.


SPECIFICITY:                         This protein binds to murine monoclonal antibodies specific for HCV NS5 protein.    NS5b protein shows strong binding to HCV converted human serum polyclonal antibodies.


BIOLOGICAL ACTIVITY:   Not determined.





Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 10  to 20-fold in PBS for solid phase antigen-antibody titration assays.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.