RODUCT#1003-G: gag p24 HIV-1 IIIB Conjugated to Colloidal Gold
Concentration: See vial Dot Blot Assay-Anti-p24 Antibody
Volume/vial: 1ml Gold Conjugate Binding
Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; Immunohistochemistr
Stability: At least 3 months at 4oC
Minimum Dilution: 1 : 50
DESCRIPTION: p24 HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size).
COUPLING RATIO: OD 520nm = 10; protein content approximately 25µg.
SPECIFICITY: Gold conjugated p24 binds to murine monoclonal antibodies of defined epitope specificity, rabbit and human polyclonal antibodies as determined by dot blot assays.
ACTIVITY: Not determined.
APPLICATIONS AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
Dot blot assays may be performed in 1 : 100 dilution range depending on the concentration of solid phase CD4/Anti-p24 in the test system.
Immunohistochemical studies may be performed in 1 : 50 dilution range.