Item#1107-G: Murine anti-vif HIV-1 Monoclonal Antibody Conjugated to Colloidal Gold
Concentration: See vial
Diluent: 50 mM Na-PO4 Buffer, pH7.4;5% BSA;0.05% PEG; 0.02% NaN3
Stability: At least 3 months at 4°C Minimum
Dilution: 1 : 50
Application: Dot Blot Assays, Immunohistocytology.
Description: High affinity murine anti-vif HIV-1 mAb conjugated to Colloidal Gold (approx. 45nm mean diameter).
Coupling Ratio: OD 520nm = 15; protein content approximately 50µg.
Specificity: Binds to native and recombinant vif HIV-1 as determined by dot blot assays.
Biological Activity: Not determined.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dot Blot assays may be performed at 10-100ng of vif in solid phase (membrane bound). Immunohistochemical studies may be performed in 1:50 to 1:100 dilution range of the gold conjugate.