PRODUCT SPECIFICATIONS
PRODUCT#1103-G: Murine Anti-p24 HIV-1 mAb Conjugated to Colloidal Gold
SPECIFICATIONS APPLICATIONS
Mass: Approximately 50µg Protein Dot Blot Assays - p24 Binding
Volume/vial: 1ml Gold Conjugate Immunohistochemistry
Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG;
0.02% NaN3
Storage: 4oC
Stability: At least 3 months at 4oC
Minimum Dilution: 1 : 100
DESCRIPTION: Murine anti-p24 HIV-1 mAb conjugated to Colloidal Gold (25nm mean particle size).
COUPLING RATIO: OD 520nm = 15; protein content approximately 50µg..
SPECIFICITY: Gold conjugated anti-p24 binds to native and recombinant p24 (HIV-1, IIIB) in dot blot assays.
BIOLOGICAL ACTIVITY: Not determined .
APPLICATIONS AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
Gold conjugated anti-p24 may be used in 1 : 100 dilution range depending on the concentration and speicificity of the test ligand (p24). Immunohistochemstry may be performed in 1 : 50 dilution range.
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