Item # 1002-F: Fluorescene tat HIV-1
Mass/vial: At least 50µg tat
Diluent: 50mM Tris, 20mM Nacl, PH8
Physical State: Frozen Liquid
F/P Ratio: 4 -5
Stability: 6 months at -75°C
Minimum Dilution: 1 : 100
Applications: Direct Immunofluorescence, Immunohistocytology, FACS, Dot Blots
Description: E.coli derived tat (HIV-1 ) conjugated with FITC.
Purification: Fluorescene conjugated tat was purified by gel exclusion chromatography and UF concentrated.
Specificity: This fluorescene conjugated tat binds to murine monoclonal antibodies of defined epitope specificity.
Biological Activity: Binding to human Hexim has not been determined.
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining may be performed in 1 : 50 dilution range. Dot Blot assays with purified anti-gp120 monoclonal/ rabbit polyclonal antibodies may be performed at a minimum dilution of 1 : 100.