Item # 1008-F: Fluorescene Recombinant HIV-1 nef
Mass/vial: At least 50µg nef
Diluent: 10mM Tris, pH 8.0
F/P Ratio: 2 - 4
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 50
Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence
Description: E.coli derived nef (HIV-1 IIIB) conjugated to FITC.
Purification: Fluorescene conjugated nef was purified by gel exclusion chromatography and UF concentrated.
Specificity: This fluorescene conjugated nef binds to murine monoclonal antibodies of defined epitope specificity.
Biological Activity: ND
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining may be performed in 1 : 50 dilution range. Dot Blot assays with purified anti-nef monoclonal/ human polyclonal antibodies may be performed at a minimum dilution of 1 : 1000