Fluorescene rgp120 HIV-1 MN (Euk)

ImmunoDXSKU: 1021-F


Product Specifications:

Item # 1021-F: Fluorescene rgp120 HIV-1 MN


Mass/vial: At least 50µg rgp120 


Diluent: 10mM Tris, pH 8.0                                  


Stabilizer: None                                                       


Preservative: None                                                       


Storage: -75°C                                                                             


F/P Ratio: 2 - 4


Stability: At least 6 months at -75°C


Minimum Dilution:   1 : 50


Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence


Description: Baculovirus derived rgp120 (HIV-1 MN) conjugated to FITC.


Purification: Fluorescene conjugated rgp120 was purified by gel exclusion chromatography and UF concentrated.


Specificity: This fluorescene conjugated rgp120 binds to murine monoclonal antibodies of defined epitope specificity and immobilized CD4 in Dot Blot assays.


Biological Activity: This fluorescene conjugated gp120 binds to CD4 bearing cells in FACS


Application and Instruction for use:

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of CD4 bearing cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified anti-gp120 monoclonal/polyclonal antibodies may be performed at a minimum dilution of 1 : 100.

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