Fluorescene rgp120 HIV-1 IIIB (Euk)

ImmunoDXSKU: 1001-F


Product Specifications:

Item # 1001-F:  Fluorescene rgp120 HIV-1 IIIB


Mass/vial: Approximately 50µg gp120                                  


Diluent: 10mM Tris, pH 8.0                                  


Stabilizer: None                                                       


Preservative: None                                                       


Storage: -75°C                                                      


Physical State: Frozen Liquid                                           


F/P Ratio: 4 -5


Stability: 6 months at -75°C


Minimum Dilution: 1 : 50


Applications: Direct Immunofluorescence, Immunohistocytology, FACS, Dot Blots


Description: Baculovirus derived rgp120 HIV-1 IIIB conjugated to FITC.


Purification: Fluorescene conjugated rgp120 was purified by gel exclusion chromatography and UF concentrated.


Specificity: This fluorescene conjugated gp120 binds to murine monoclonal antibodies of defined epitope specificity.


Biological Activity: This fluorescene conjugated gp120 binds to CD4 bearing T-cells  as determined by  FACS.


Application and Instruction for use:


Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining may be performed in 1 : 50 dilution range.  Dot Blot assays with purified anti-gp120 monoclonal/ rabbit polyclonal antibodies may be performed at a minimum dilution of 1 : 100.


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