Item # 1001-F: Fluorescene rgp120 HIV-1 IIIB
Mass/vial: Approximately 50µg gp120
Diluent: 10mM Tris, pH 8.0
Physical State: Frozen Liquid
F/P Ratio: 4 -5
Stability: 6 months at -75°C
Minimum Dilution: 1 : 50
Applications: Direct Immunofluorescence, Immunohistocytology, FACS, Dot Blots
Description: Baculovirus derived rgp120 HIV-1 IIIB conjugated to FITC.
Purification: Fluorescene conjugated rgp120 was purified by gel exclusion chromatography and UF concentrated.
Specificity: This fluorescene conjugated gp120 binds to murine monoclonal antibodies of defined epitope specificity.
Biological Activity: This fluorescene conjugated gp120 binds to CD4 bearing T-cells as determined by FACS.
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining may be performed in 1 : 50 dilution range. Dot Blot assays with purified anti-gp120 monoclonal/ rabbit polyclonal antibodies may be performed at a minimum dilution of 1 : 100.
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