Item # 1081-F: Fluorescene rgp120 HIV-1 ADA
Mass/vial: At least 50µg gp120
Diluent: 10mM Tris, pH 8.0
F/P Ratio: 2 - 3
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 50
Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence
Description: Baculovirus derived rgp120 (HIV-1 ADA) conjugated to FITC.
Purification: Fluorescene conjugated rgp120 was purified by gel exclusion chromatography and UF concentrated.
Specificity: This fluorescene conjugated rgp120 binds to murine monoclonal antibodies of defined epitope specificity and immobilized CD4 in Dot Blot assays.
Biological Activity: This fluorescene conjugated gp120 binds to CD4 bearing cells in FACS.
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of CD4 bearing cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified anti-gp120 monoclonal/polyclonal antibodies may be performed at a minimum dilution of 1 : 100.
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