Fluorescene rgp120 HIV-1 ADA

SKU: 1081-F
Size: 50 ul

Product Specifications:

Item # 1081-F: Fluorescene rgp120 HIV-1 ADA


Mass/vial: At least 50µg gp120


Diluent: 10mM Tris, pH 8.0                                  


Stabilizer: None                                                       


Preservative: None                                                       


Storage: -75°C                                                                             


F/P Ratio: 2 - 3


Stability: At least 6 months at -75°C


Minimum Dilution:   1 : 50


Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence


Description: Baculovirus derived rgp120 (HIV-1 ADA) conjugated to FITC.


Purification: Fluorescene conjugated rgp120 was purified by gel exclusion chromatography and UF concentrated.


Specificity: This fluorescene conjugated rgp120 binds to murine monoclonal antibodies of defined epitope specificity and immobilized CD4 in Dot Blot assays.


Biological Activity: This fluorescene conjugated gp120 binds to CD4 bearing cells in FACS.


Application and Instruction for use:

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of CD4 bearing cells may be performed in 1 : 50 dilution range.  Dot Blot assays with purified anti-gp120 monoclonal/polyclonal antibodies may be performed at a minimum dilution of 1 : 100.