Item # 1302-F: Fluorescene Rabbit Anti-tat HIV-1 IIIB pAb IgG
Mass/vial: Approx. 50ug IgG
F/P Ratio: 2 - 4
Physical State: Frozen Liquid
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 50
Applications: Immunohistocytology, Immunostaining/sorting, Dot Blots, FACS.
Description: Fluorescene conjugated anti-tat (HIV-1 IIIB) pAb IgG.
Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This fluorescene conjugated pAb IgG binds to native and recombinant HIV-1 tat in Dot Blot assays and stains cells in direct immunofluorescence assays.
Biological Activity: None
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified gp120 may be performed at a minimum dilution of 1 : 100.