Item # 1108-F: Fluorescene Murine anti-nef HIV-1Monoclonal Antibody
Antibody class: IgG1
Diluent: 10mM Tris, pH 8.0
F/P Ratio: 3 - 4
Physical State: Frozen Liquid
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 100
Applications: Immunohistocytology, Immunostaining/sorting, Dot Blots, FACS.
Description: R-Phycoerythin conjugated Murine Anti-vif HIV-1 mAb (clone 15).
Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This R-Phycoerythrin conjugated mAb binds to native and recombinant HIV-1 vif as determined by dot blot and Western blot assays. Detection range: 50-200pg vif.
Biological Activity: None
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dot Blot assays with purified vif may be performed in 1 : 200 dilution range. vif may be detected in viral lysates by direct immunofluorescent assays. R-Phycoerythrin conjugates can be used with three- and four-color fluorescence cell sorting. R-Phycoerythrin is excited with argon ion laser at 488nm and detected at 570nm.
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