Item# 80002: Recombinant Chikungunya E2 Antigen (E.coli)
Concentration: See vial
Mass/vial : See vial
Diluent : 0.1M NaCl, 50mM Tris, pH8, 5mM DTT, 0.02%SDS.
Purity : >95%
Physical State: Frozen Liquid
Stability: At least 36 months at -750C.
Applications: Elisa/Western Blots,Diagnostics.
Description: Synthetic Chikungunya virus E2 DNA sequence, amino acid residues 1-352 , expressed in the E.coli Expression System.
Purification: This protein is purified by aqueous solvent extraction and preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 41.1kD.
Specificity: This protein binds Chikungunya virus-converted human serum polyclonal antibodies as determined by Elisa, Western Blots and one-step lateral flow diagnostic testing.
Application and Instructions for use
Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for tittering Chikungunya virus converted human polyclonal antibodies. Concentrations of 1-2mg/ml antigen (1-2ul/cm) are recommended for Lateral Flow Diagnostics testing.