Item# 20012: Recombinant T. cruzi (Chagas) Microtubule-Associated Protein, MAP
Concentration: See vial
Mass/vial: See vial
Volume/vial: See vial
Diluent: 20mM NaPO4, 10mM NaCl, pH 8
Physical State: Frozen Liquid (-75°C), Lyophilized (RT)
Stability: 3 years
Application: In-Vitro Diagnostics, Immunization.
Description: Recombinant T. cruzi (Chagas) Microtubule-Associated Protein, MAP produced in the Baculovirus Expression System.
Purification:This protein is purified by Ni-affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
Approx. Molecular Weight:20.1kD.
Specificity: This protein binds to T. cruzi (Chagas) converted human serum polyclonal antibodies in ELISA and Western Elisa. Qualified for one-step immunochromatographic diagnostic tests.
Biological Activity: Not done
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml, 1ul/cm.
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