Item# 20014: Recombinant T. cruzi (Chagas) Composite Antigen A13,B13,H49,1F8 Antigen Baculo)
Concentration: See vial
Mass/vial: See vial
Volume/vial: See vial
Diluent: 20mM NaPO4, 10mM NaCl, 0.05% Sark, pH8 .
Physical State: Frozen Liquid (-75°C), Lyophilized (RT)
Stability: 3 years
Application: In-Vitro Diagnostics, Immunization.
Description: Recombinant T. cruzi (Chagas) combination A13, B13, H49 and 1F8 antigen expressed in the Baculovirus Expression System.
Purification:This protein is purified by affinity chromatography and by preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.
Approx. Molecular Weight: 84kD.
Specificity: This protein binds to T. cruzi (Chagas) converted human serum polyclonal antibodies in ELISA and Western Elisa. Qualified for one-step immunochromatographic diagnostic tests.
Biological Activity: Not done
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml, 1ul/cm.
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