Item# 1003-B: Biotin p24 HIV-1 IIIB
Vol./Vial: 50ul,50ug protein
Diluent: 50mM Dodium Phosphate, 10mM NaC1,pH7.5
Stability: 12 months at 4°C
Minimum Dilution: 1 : 1000
Application: T-Cell Research, Diagnostics, Drug Screening, ELISA, Western ELISA
Description: Biotin conjugated to recombinant (baculovirus) HIV-1p24.
Coupling Ratio: Approximately 10-20 moles of biotin per mole p24.
Specificity: Biotin p24 binds to anti-p24 monoclonal and polyclonal antibodies in Elisa and western Elisa as determined by strepavidin-AP/HRP assays.
Biological Activity: Biotin p24 binds to solid phase anti-p24 pAb (oligoclonal) as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA may be performed in 1 : 5000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. p24/Anti-p24 pAb capture ELISA may be performed in 1-10ng pAb in solid phase and 0.1-1ng of biotin-p24.