Item# 1021-B: Biotin rgp120 HIV-1 MN
Diluent: PBS + 0.2% BSA
Stability: Not Determined
Minimum Dilution: 1 : 2000
Applications: T-Cell Research, ELISA, Western ELISA, Diagnostics, Drug Screening.
Description: Biotin conjugated to rgp120 HIV-1 MN.
Coupling Ratio: Approximately 10-20 moles of biotin per mole gp120.
Specificity: Biotin gp120 MN binds to Murine anti-gp120 mAbs (structural and non-structural epitopes) as determined by biotin- strepavidin-AP/HRP ELISA and Western ELISA assays.
Biological Activity: Biotin gp120 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Application and Instructions for use:
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA may be performed in 1 : 10,000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. gp120/CD4 capture ELISA may be performed in 100ng CD4 in solid phase and .1-1.0ng of biotin-gp120.
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