Item# 1301-B: Biotin-Conjugated Rabbit Anti-gp120 pAb
Stabilizer: 2.5% BSA
Preservative: 0.01% NaN3
Physical State: Liquid
B/P Ratio: 10 - 20
Stability: At least 3 months at 4°C
Minimum Dilution: 1 : 2000
Applications: ELISA, Westerns, Immunohistocytology, gp120 Capture Assays, Drug Screening, Human T-cell Research
Description: Biotin conjugated Rabbit anti-gp120 pAb.
Specificity: Biotin conjugated Rabbit anti-gp120 pAb binds to native and recombinant gp120 (V3 loop specific) in ELISA and Western ELISA.
Biological Activity: Biotin conjugated Rabbit anti-gp120 pAb binds to gp120 in CD4/gp120 capture ELISA and Western ELISA.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA and Western ELISA may be performed at dilutions of 1 : 2000 depending on the concentration of gp120 in the test system. CD4/gp120 capture ELISA may be performed in 1 : 500 dilution range depending on the capture ligand CD4 concentration and gp120 concentration in the test sample. Typical assays with 100ng of solid phase CD4 will detect at least 10ng/ml gp120.