PRODUCT # 1107 -B: Biotin Murine Anti-vif HIV-1 mAb IgG
Mass/vial: Approximately 50µg IgG Elisa/Westerns
Volume: 50µl CEM15/Vif Elisa
Diluent: PBS Immunohistochemistry
Stabilizer: 1% BSA
Preservative: 0.05% Azide
Physical State: Liquid
B/P Ratio: 10 - 20
Stability: At least 12 months at 4oC.
Minimum Dilution: 1 : 2000
DESCRIPTION: Biotin conjugated Murine anti-vif HIV-1 mAb (clone 15).
PURIFICATION: Highly purified mAb IgG (>95% pure) was used for biotin conjugation and the conjugate was purified by gel exclusion chromatography.
SPECIFICITY: This biotin conjugated mAb binds to native and recombinant HIV-1 vif in Dot Blot assays, ELISA and Western ELISA. Useful for CEM15/vif binding assays.
BIOLOGICAL ACTIVITY: None
APPLICATION AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg p66 range with 1:500 dilution of the conjugate.