Item # 1106-B: Biotin Murine Anti-p66 (RT) HIV-1 mAb IgG
Mass/vial: Approx 50µg IgG
Stabilizer: 1% BSA
Preservative: 0.1% Azide
Physical State: Liquid
B/P Ratio: 10 - 20
Stability: At least 6 months at 4°C
Minimum Dilution: 1 : 5000
Applications: ELISA/ Western ELISA, FACS, Immunohistocytology,
Description: Biotin conjugated Murine anti-p66 (RT) HIV-1 mAb.
Purificatiion: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 p66 in Dot Blot assays, ELISA and Western ELISA.
Biological Activity: None
Application and Instructins for use
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg p66 range with 1:500 dilution of the conjugate.