Item# 1103-B: Biotin Murine Anti-p24 HIV-1 IIIB mAb IgG
Mass/vial: Approximately 50µg IgG
Preservative: 0.02% Azide
Physical State: Liquid
B/P Ratio: 10 - 20
Stability: At least 6 months at 4°C
Minimum Dilution: 1 : 2000
Application: Dot Blots, FACS, Immunohistocytology, Direct Immunofluorescence.
Description: Biotin conjugated Murine anti-p24 (HIV-1 IIIB) mAb.
Purification: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 p24 in Dot Blot assays, ELISA and Western ELISA.
Biological Activity: None
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg p24 range with 1-2µg/ml conjugate.
Item# 1103: Murine mAb p24 HIV-1 IIIB
Application: ELISA, Western ELISA, Immunoprecipitation, Immunohistochemistry.
Item# 1103-P: Peroxidase-Conjugated Murine mAb p24 HIV-1 IIIB
Application: Diagnostics, ELISA, Western ELISA.
Item# 1103-G: Colloidal Gold-Conjugated Murine mAb p24 HIV-1 IIIB
Application: Diagnostics, Dot Blot, Histocytology.