Biotin Murine mAb nef HIV-1

SKU: 1108-B
Size: 50 ug

Product Specifications:

Item # 1108-B: Biotin Murine Anti-Nef HIV-1 mAb IgG


Mass/vial: Approximately 50µg IgG 


Volume: 50µl 


Diluent: PBS


Stabilizer: 1% BSA


Preservative: 0.1% Azide


Storage: 4°C


Physical State: Liquid 


B/P Ratio: 5-10


Stability: At least 6 months at 4°C


Minimum Dilution: 1 : 2000


Application: FACS, ELISA, Westerns, Immunohistocytology.


Description: Biotin conjugated Murine anti-Nef HIV-1 mAb.


Purification: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.


Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 Nef in ELISA and Western ELISA.


Biological Activity: None


Application and Instructions for use:


Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg Nef range with 1:500 dilution of the conjugate.