Biotin Murine mAb nef HIV-1

$360.00
SKU: 1108-B
Size: 50 ug

Product Specifications:

Item # 1108-B: Biotin Murine Anti-Nef HIV-1 mAb IgG

 

Mass/vial: Approximately 50µg IgG 

 

Volume: 50µl 

 

Diluent: PBS

 

Stabilizer: 1% BSA

 

Preservative: 0.1% Azide

 

Storage: 4°C

 

Physical State: Liquid 

 

B/P Ratio: 5-10

 

Stability: At least 6 months at 4°C

 

Minimum Dilution: 1 : 2000

 

Application: FACS, ELISA, Westerns, Immunohistocytology.

 

Description: Biotin conjugated Murine anti-Nef HIV-1 mAb.

 

Purification: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.

 

Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 Nef in ELISA and Western ELISA.

 

Biological Activity: None

 

Application and Instructions for use:

 

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg Nef range with 1:500 dilution of the conjugate.