PRODUCT # 1108-B: Biotin Murine Anti-Nef HIV-1 mAb IgG
Mass/vial: Approximately 50µg IgG Elisa/Westerns
Volume: 50µl FACS
Diluent: PBS Immunohistocytology
Stabilizer: 1% BSA
Preservative: 0.1% Azide
Physical State: Liquid
B/P Ratio: 5-10
Stability: At least 6 months at 4oC.
Minimum Dilution: 1 : 2000
DESCRIPTION: Biotin conjugated Murine anti-Nef HIV-1 mAb.
PURIFICATION: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.
SPECIFICITY: This biotin conjugated mAb binds to native and recombinant HIV-1 Nef in ELISA and Western ELISA.
BIOLOGICAL ACTIVITY: None
APPLICATION AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg Nef range with 1:500 dilution of the conjugate.