Item# 1121-B: Biotin Murine Anti-gp120 HIV-1 mAb IgG
Mass/vial: Approximately 50µg IgG
Stabilizer: 2.5% BSA
Preservative: 0.02% Azide
Physical State: Liquid
B/P Ratio: 10 - 20
Stability: At least 6 months at 4°C
Minimum Dilution: 1 : 5000
Applications: ELISA, Westerns, FACS, Immunohistopcytology.
Description: Biotin conjugated Murine anti-gp120 (HIV-1) cross-reactive mAb.
Purification: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 gp120 in Dot Blot assays, ELISA and Western ELISA.
Biological Activity: None
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 5000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 1-10pg gp120 range with 0.1-0.5µg/ml conjugate.
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