Biotin Murine Anti-gp120 YU2

ImmunoDXSKU: 1131-B



Product Specifications:

Item# 1131-B: Biotin Murine Anti-gp120 HIV-1 YU2 mAb IgG


Mass/vial: Approximately 50µg IgG 


Volume: 50µl  


Diluent: PBS


Stabilizer: 2.5% BSA


Preservative: 0.02% Azide


Storage: 4°C


Physical State: Liquid


B/P Ratio: 10 - 20


Stability: At least 6 months at 4°C


Minimum Dilution: 1 : 5000


Applications: ELISA, Westerns, FACS, Immunohistopcytology.


Description: Biotin conjugated Murine anti-gp120 (HIV-1) YU2 M Tropic mAb


Purification: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.


Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 gp120 YU2 M Tropic and HIV-1 MN ( Dual Tropic ), but not to HIV-1 ADA M Tropic, in Dot Blot assays, ELISA and Western ELISA.


Biological Activity: None


Application and Instructions for use: 

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 5000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg gp120 range with 0.1-0.5µg/ml conjugate.

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