Item# 1001-BL: Biotin rgp120 HIV-1 IIIB
Diluent: PBS+ 0.2% BSA, pH 7.5
Preservative: 0.1% NaN3
Stability: 6 Months
Minimum Dilution: 1 : 2,000
Application: T-Cell Research, Diagnostics, Drug Screening, ELISA, Western ELISA.
Description: Biotin conjugated to rgp120 HIV-1 IIIB.
Coupling Ratio: Approximately 5-20 moles of biotin per mole gp120.
Specificity: Biotin gp120 binds to Murine and Human Anti-gp120 mAbs as determined by strepavidin-HRP ELISA and Western ELISA assays.
Biological Activity: Biotin gp120 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA may be performed in 1 : 10,000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. gp120/CD4 capture ELISA may be performed in 100ng CD4 in solid phase and 0.1-10ng of biotin-gp120.