Item# 105-30: Recombinant M Tuberculosis 38 kD Antigen (E.coli)
Concentration: See vial
Volume/vial: See vial
Diluent: 50mM NaPO4, pH 7.0 0.1M NaCl , 0.05% SOD. Sarcosyl
Physical State: Liquid, Sterile filled
Stability: 2 year at -75°C
Applications: ELISA, Western ELISA, TB Diagnostics
Descri[ption: M tuberculosis 38 kD Antigen produced in the E. coli expression system.
Purificatiuon: Purified by ion affinity and UF concentration solvent extraction to >98% purity as determined by SDS-PAGE.
Molecular Weight: approx. 38.9kD
Specificity: This 38 kD protein reacts with human TB serum in ELISA and Western ELISA.
Biological Activity: Not done
Application and Instructions for use:
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.