38kD Antigen

$1,295.00
SKU: 105-30

Product Secifications:

Item# 105-30: Recombinant M Tuberculosis 38 kD Antigen (E.coli)

 

Concentration: See vial

 

Mass/vial: 1mg

 

Volume/vial: See vial

 

Diluent: 50mM NaPO4, pH 7.0 0.1M NaCl , 0.05% SOD. Sarcosyl

 

Purity: >98%

 

Stabilizer: None

 

Preservative: None

 

Storage: -75°C

 

Physical State: Liquid, Sterile filled

 

Stability: 2 year at -75°C

 

Applications: ELISA, Western ELISA, TB Diagnostics

 

Descri[ption: M tuberculosis 38 kD Antigen produced in the E. coli expression system.

 

Purificatiuon: Purified by ion affinity and UF concentration solvent extraction to >98% purity as determined by SDS-PAGE.

 

Molecular Weight: approx. 38.9kD

 

Specificity: This 38 kD protein reacts with human TB serum in ELISA and Western ELISA.

 

Biological Activity: Not done

 

Application and Instructions for use: 

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.