Rabbit anti MPT64pAb

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ImmunoDXSKU: 135-40

Stock: IN STOCK
Price:
$420
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PRODUCT # 135-40:  Rabbit Anti-rTB MPT64 Antigen Polyclonal Antibodies


SPECIFICATIONS APPLICATIONS

Concentration: 1mg/ml MPT64 ELISA

Mass/vial: 0.1mg/100ul MPT64 Western ELISA

Diluent: PBS Immunoprecipitation

Purity: >95% Human TB Diagnostics

Stabilizer: None

Preservative: None

Storage: -75oC

Physical State: Frozen Liquid

Stability: At least 24 months

at -70oC

Minimum Dilution: 1 : 1000


DESCRIPTION: pAb IgG fraction purified from rTB MPT64 antigen converted rabbit immune serum.


PURIFICATION: This pAb IgG was purified by protein A/G affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.


SPECIFICITY: This pAb IgG binds to native and recombinant TB antigen MPT64 as determined by ELISA and Western ELISA. Useful in capturing MPT64 specific polyclonal antibodies from human active TB serum in a twin site assay using anti-MPT64 murine monoclonal antibody (product # 115-24) and rabbit anti-MPT64 polyclonal antibody-HRP as a detection reagent ( product # 135-20-P).

BIOLOGICAL

ACTIVITY: None


APPLICATION AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.


This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized rESAT-6(Product# 135-40) under standard ELISA conditions.  ELISA and Western ELISA may be performed at 1-2µg/ml of pAb depending on the nature and the quantity of the immobilized antigen.  


RELATED PRODUCTS

Product#135-40-P: Peroxidase-Conjugated Rabbit anti-MPT64 pAb

Product #145-40:             Goat anti-MPT64 Polyclonal Antibody

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