Recombinant M Tuberculosis CFP-10 Antigen (E.coli)

ImmunoDXSKU: 105-20


Product Specifications:

Item# 105-20: Recombinant M Tuberculosis CFP-10 Antigen (E.coli) 

Concentration: 2mg/ml

Mass/vial: 1mg/vial 

Volume/vial: 500ul 

Diluent: 50mM NaPO4, pH 7.0 0.1M NaCl , 0.05% SOD. 

Purity: >98%

Stabilizer: None

Preservative: None

Storage: -75°C

Physical State: Frozen Liquid

Stability: Not Determined

Description: M. Tuberculosis (virulent strain specific) CFP-10 antigen produced in the E. coli expression system.

Purificatiuon: Purified by ion affinity, solvent extraction and UF concentrated to >98% purity as determined by SDS-PAGE, reduced.

Molecular Weight: approx. 11kD

Specificity: This CFP-10 protein reacts with human TB serum in ELISA and Western ELISA. Suitable for diagnostic applications.

Biological Activity: Not Determined

Application and Instructions for use: 

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. CAUTION: This product is sterile filtered and is stable at refrigeration temperature. Remove product aseptically from the vial.

Articles related to CFP-10:

Upon infection with Mycobacterium tuberculosis, neutrophils are massively recruited to the lungs, but the role of these cells in combating the infection is poorly understood. Through a type VII secretion system, M. tuberculosis releases a heterodimeric protein complex, containing a 6-kDa early secreted antigenic target (ESAT-6) and a 10-kDa culture filtrate protein (CFP-10), that is essential for virulence. Whereas the ESAT-6 component possesses multiple virulence-related activities, no direct biological activity of CFP-10 has been shown, and CFP-10 has been described as a chaperone protein for ESAT-6. We here show that the ESAT-6:CFP-10 complex induces a transient release of Ca2+ from intracellular stores in human neutrophils. Surprisingly, CFP-10 rather than ESAT-6 was responsible for triggering the Ca2+ response, in a pertussis toxin-sensitive manner, suggesting the involvement of a G-protein-coupled receptor. In line with this, the response was accompanied by neutrophil chemotaxis and activation of the superoxide-producing NADPH-oxidase. Neutrophils were unique among leukocytes in responding to CFP-10, as monocytes and lymphocytes failed to produce a Ca2+ signal upon stimulation with the M. tuberculosis protein. Hence, CFP-10 may contribute specifically to neutrophil recruitment and activation during M. tuberculosis infection, representing a novel biological role for CFP-10 in the ESAT-6:CFP-10 complex, beyond the previously described chaperone function.


Payment & Security

American Express Apple Pay Diners Club Discover Meta Pay Google Pay Mastercard Shop Pay Visa

Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.

Estimate shipping

You may also like