Item#1017: Recombinant HIV-1 vif (Baculovirus)
Concentration: See vial
Volume/vial: See vial
Diluent: 0.1M Na-Ac, pH 6.5, 1mM DTT
Physical State: Frozen Liquid
Stability: Minimum 12 months at -75°C
Application: ELISA, Western ELISA, Immunological Studies, Drug Screening.
Description: Full length Recombinant HIV-1 HXB2 vif produced as a GST-fusion protein in Baculovirus
Purification: This recombinant protein is purified by GST -Affinity chromatography to >95% purity as determined by SDS-PAGE reduced.
Approx. Molecular Weight: 45kD.
Specificity: This protein shows reactivity with murine monoclonal antibodies and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: Vif biding to APOBEC3G (CEM15) as determined by ELISA.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the test antibody.