rvif HIV-1 IIIB (Baculovirus)

SKU: 1017

Product Specifications:

Item#1017: Recombinant HIV-1 vif (Baculovirus)

 Concentration: See vial

 Mass/vial: 100ug

 Volume/vial: See vial

 Diluent: 0.1M Na-Ac, pH 6.5, 1mM DTT

 Purity: >95%

 Stabilizer: None

 Preservative: None

 Storage: -75°C

 Physical State: Frozen Liquid

 Stability: Minimum 12 months at -75°C

 Application: ELISA, Western ELISA, Immunological Studies, Drug Screening.

 Description:   Full length Recombinant HIV-1 HXB2 vif produced as a GST-fusion protein in Baculovirus

 Purification: This recombinant protein is purified by GST -Affinity chromatography to >95% purity as determined by SDS-PAGE reduced. 

 Approx. Molecular Weight: 45kD.

 Specificity: This protein shows reactivity with murine monoclonal antibodies and HIV-1 converted human serum polyclonal antibodies in ELISA and Western ELISA.

 Biological Activity: Vif biding to APOBEC3G (CEM15) as determined by ELISA.

 Application and Instructions for use

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng  protein depending on the nature and affinity of the test antibody.