rp66 (RT) HIV-1 IIIB (E. coli)

SKU: 1006

Product Specifications:

Item# 1006-10: Recombinant HIV-1 IIIB p66 (RT)

 Concentration: See vial

 Mass/vial: 1mg

 Volume/vial: See vial

 Diluent: 50mM Tris,pH8,10% Glycerol 1mM DTT,0.2M NaCl

 Purity: >95%

 Stabilizer: None

 Preservative: None

 Storage: -75°C

 Physical State: Frozen Liquid

 Stability: At least 24 months at -75°C.

 Application: ELISA, Western ELISA, Reverse Trascriptase Reference, Drug Screening.

Description: Full length Recombinant heterodimeric HIV-1 IIIB p66 (RT) produced in E.coli expression system.

 Purification: This heterodimeric protein is purified by ion-affinity chromatography to >98% purity as determined by SDS-PAGE, reduced: apparent MW= 66kD.

 Specificity: This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA.

Biological Activity: 0.1 unit of purified RT incorporates a minimum of 5 picomoles of dTTP per minute under standard RT assay conditions.

Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody. Solid phase antigen in 50-100ng amounts shows reactivity with human serum antibodies in dot blot assays.