rp22 Capsid HCV (Euk)

$435.00
SKU: 4003
Size: 100 ug

PRODUCT SPECIFICATIONS

Product # 4003:  Recombinant HCV 22kD Core Protein (Baculo)

                                                           

           

                                                             

  Concentration:                 1mg/ml                                                           

  Mass/vial:                        100ug                                                                             

  Diluent:                            50mM Tris,pH8,0.15M NaCl

                                           6M Urea.                                                           

  Purity  :                            >95%                                                                      

  Stabilizer:                         None                                                                       

  Preservative:                     None                                                                     

  Storage:                            -75o C

  Stability:                           At least 12 months at -75o C

  Applications:                    In-Vitro HCV Diagnostics,     T-Cell Activation                                                 

  Description:                    Hepatitis C full length  nucleocapsid recombinant proretn (p22) produced                                                                  in Pichia  pastoris expression system.

                                       

 

 Purification:                 HCV core protein is purified by immune-affinity chromatography methods                                                                to >95% purity as determined by SDS-PAGE, reduced. Mol Wt: 16kD.

 

 Specficity:                   This protein binds murine monoclonal antibodies and HCV converted human                                                            serum polyclonal antibodies in ELISA and Western  ELISA.

 

 Biological Activity:      Not determined.

 

 

APPLICATION AND INSTRUCTIONS FOR USE

 

Recommended concentrations for use are approximate values. Dilute the antigen preparation at least 10  to 20-fold in PBS for solid phase antigen-antibody titration assays.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

 HCV core protein shows relatively strong binding specificity for HCV-specific human antibodies. ELISA and Western ELISA require protein in 10ng range depending on the nature and affinity of the secondary detection reagent. HCV converted human serum polyclonal antibodies yield titers of 1:10, 000 or greater at 100ng of immobilized protein under standard ELISA conditions.