Item# 5008: Recombinant SIVmac251 nef (Baculovirus)
Concentration: See vial
Diluent: 50mM Tris, pH8, 150mM NaCl
Purity : >95%
Physical State: Frozen Liquid
Stability: At least 6 months at -75°C.
Applications: Cell Activation,Immunological Studies, Drug Screening, ELISA / Western ELISA, Diagnostics
Description: Full length Recombinant SIV mac251 nef produced as a GST fusion protein in the Baculovirus Expression System.
Purification: This recombinant protein is purified by Glutathione -affinity chromatography and the purified protein is thrombin-cleaved to produce nef of >95% purity, as determined by SDS-PAGE, reduced.
Specificity: This protein shows reactivity with murine monoclonal antibodies and SIV converted serum polyclonal antibodies in ELISA and Western ELISA.
Biological activity: Not done.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.