Item# 7001 Recombinant Human T-Cell Receptor sCD4
Concentration: See vial
Physical State: Frozen Liquid
Stability: At least 24 months at -75°C
Applications: T-Cell Research,Human Diagnostics, HIV-1 Neutralization, Drug Screening
Description: Full length recombinant human T-Cell receptor sCD4 produced in the baculovirus expression system. The full length protein contains V1 through V3 domains of CD4.
Purification:This protein is purified by immunoaffinity and gel exclusion chromatography to >98% purity as determined by SDS PAGE, reduced.
Molecular weight: Approximately 55kD.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western ELISA.
Biological Activity: This protein binds to native and recombinant HIV-1 gp120 and HIV-2 gp105 and neutralizes HIV-1 infectivity in CD4-bearing, HIV-1 infected cell cultures and HIV infected PCMBs.
Application and Instructions for use:
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s).
2. Recommended concentration for virus neutralization assays are in 1-10µg/ml of the HIV-1 infected culture.
3. Drug screening assays are performed with 100-500ng of solid phase sCD4 and gp120 binding is determined in the presence of inhibitors either indirectly using murine anti-gp120-peroxidase conjugate (catalog# 1121-P) or directly using HIV-1 rgp120-peroxidase conjugate (catalog# 1001-P).