Item# 1011: Recombinant HIV-1 C Envelope Glycoprotein gp120 (Baculovirus)
Concentration: See Vial
Physical State: Frozen Liquid
Stability: At least 2 years at -75oC.
Applications: Vial In-Vitro Diagnostics, T-Cell Activation, CD4 Binding.
Description: Full length Recombinant HIV-1 C gp120 glycoprotein produced in the Baculovirus Expression System.
Purification: This protein is purified by immune-affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent Cells.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. HIV-converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 1-10ng of immobilized protein under standard ELISA conditions.