rgp41 SIV (Baculo)

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ImmunoDXSKU: 5019

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PRODUCT# 5019:  Recombinant SIV gp41 Envelope Protein (E.coli)

                                                           

  SPECIFICATIONS                                                            

  Concentration:                 See vial                                                   

  Mass/vial:                          0.1mg                                                    

   Volume/vial:                     see vial                                                             

  Diluent:                           50 mM Tris, 0.1M NaCl, 2mM DDT,pH8                                                                    

  Purity:                              >98%                                                                                                    

  Stabilizer:                          None                                                                        

  Preservative:                    None                                                                     

  Storage:                             -75oC

  Physical State                Frozen Liquid

  Stability:                            At least 24 months at -75 oC.                          

                                              

DESCRIPTION:  Truncated Recombinant SIV gp41 envelope protein produced in the E.coli Expression System. Truncated gp41 protein mol.wt: 28kD.

 

PURIFICATION: This protein was purified by solvent extraction and and by preparative  electrophoresis to >98% purity as determined by SDS-PAGE, reduced.                                                                                            

SPECIFICITY: This protein binds to HIV-2 converted human serum polyclonal antibodies in ELISA and Western  Elisa.

BIOLOGICAL ACTIVITY:  Not done

APPLICATION AND INSTRUCTIONS FOR USE

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

 ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.

Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard

ELISA conditions.

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