Item# 60011: Recombinant Toxoplasma GRA1,3,6,7, M2AP Antigen (Baculo)
Concentration: See vial
Diluent: 50mM Tris,20mM Nacl, SDS trace, pH8+-
Storage: long term -75°C, Short term-20C°
Physical State:Frozen Liquid
Stability: 2 years at -75°C
Application: In-Vitro Diagnostics, Immunization
Description: Recombinant Toxoplasma GRA1,3,6,7, M2AP Antigen produced in the Baculovirus Expression System.
molecular Weight: 62kD.
Purification: This protein is purified by solvent extraction and preparatice electrophoresisi to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to Toxoplasma converted human serum polyclonal antibodies (IgG,IgM) in ELISA and Western Elisa, Dot Blots and Lateral Flow immunochromatographic diagnostic tests.
Biological Activity: Not done
Applications and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml.