T. pallidum TP15 (E.coli)

ImmunoDXSKU: 15001


Product Specifications:

Item# 15005:  Recombinant Treponema pallidum 17kD Membrane Protein (E.coli)


Concentration: 1mg/ml                                                               




Volume/vial: 1ml                                                            


Diluent: 10 mM Tris, pH 8.0, SDS (<0.01%)                                                                  


Purity:  >95%                                                                                                    


Stabilizer:   None                                                                        


Preservative: None                                                                     


Storage:  -75°C


Physical State:Frozen Liquid


Stability:  2 years at -75°C 


Application: In-Vitro Diagnostics, Immunization


Description: Recombinant Treponema pallidum 17Kd protein produced as a gst-fusion protein in the E.coli Expression System. Fusion protein protein


molecular Weight: 42kD.


Purification: This protein is purified by affinity chromatography and by preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.


Specificity: This protein binds to murine anti-TP17 kD protein monoclonal antibodies and T. pallidum converted human serum polyclonal antibodies in ELISA and  Western Elisa, Dot Blots and Lateral Flow immunochromatographic diagnostic tests.


Biological Activity:  Not done


Applications and Instructions for use


Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml.

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