Product Specifications:
Item# 90020: Recombinant LeptospiraComposite L2 Antigen OmpL1-MLP17-LigA (E.coli)
Concentration: See Vial
Mass/vial: See vial
Volume/vial: See Vial
Diluent: 50mM Tris, 50mM NaCl, pH9, 10%Glycerol
Purity: Approx. 98%
Stabilizer: None
Preservative:None
Storage: -75°C
Physical State: Frozen Liquid
Stability: 2 years at -75°C
Application: In-Vitro Diagnostics, Immunization.
Description:Full length Recombinant Leptospira Composite L2 antigen- LipL32-LipL41-produced in the E.coli Expression System.
Purification:This protein is purified by solvent extraction, preparative electrophoresis and fusion tag affinity immuno-chromatography (proprietary) to >98% purity, as determined by SDS-PAGE, reduced
Approx. Molecular Weight: 62.2kD.
Specificity: This protein binds to Leptospira converted human serum polyclonal antibodies, as determined by ELISA and Western ELISA. Suitable for use in lateral flow human Leptospira diagnostics.
Biological Activity: Not Determined.
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Leptospira converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.
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