Item# 80001: Recombinant Chikungunya E1 Antigen (E.coli)
Concentration: See vial
Diluent :0.1mM NaCl, 50mM Tris, pH8, 5mM ddt, 0.02%SDS
Physical State: Frozen Liquid
Stability: At least 36 months at -750C
Applications: Elisa/Western Blots, Diagnostics.
Description: Synthetic Chikungunya virus E1 DNA sequence, amino acid residues 66-430 , expressed in the E.coli Expression System
Purification: This protein is purified by aqueous solvent extraction and preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
Molecular Weight: approx. 41kD.
Specificity: This protein binds to Chikungunya virus-converted human serum polyclonal antibodies as determined by Elisa, Western Blots and by one-step lateral flow diagnostic testing.
Application and Instructions for use
Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml antigen range for tittering Chikungunya virus converted human polyclonal antibodies. Concentrations if 1-2mg/ml antigen (1-2ul/cm) are recommended for Lateral Flow Diagnostics.
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