Item# 20001: Recombinant T. cruzi (Chagas) Complement Regulatory Protein, CRP (Prokaryotic)
Concentration: See Vial
Mass/vial: See vial
Volume/vial: See Vial
Diluent: PBS, 5% glycerol
Physical State: Frozen Liquid
Stability: 2 years at -75°C
Application: In-Vitro Diagnostics, Immunization.
Description: Recombinant T. cruzi (Chagas) Complement Regulatory Protein, CRP produced in the E.coli Expression System.
Purification:This protein is purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
Approx. Molecular Weight: 107.9kD.
Specificity: This protein binds to T. cruzi (Chagas) converted human serum polyclonal antibodies in ELISA and Western Elisa. Qualified for one-step immunochromatographic diagnostic tests.
Biological Activity: Not done
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml, 1ul/cm.