Item# 20004: Recombinant T. cruzi (Chagas) Composite Antigen A13, B13,H49,1F8 Antigen (E.coli)
Mass/vial: 1mg/ml, ml
Diluent: 50mM Tris, 50mM NaCl, pH 8.3, SDS trace
Storage: Short term -20°C, long term -75°C
Physical State: Frozen Liquid
Stability: 2 years at -75°C
Application: In-Vitro Diagnostics, Immunization.
Description: Recombinant T.cruzi (Chagas) combination A13, B13, H49 and 1F8 antigen expressed in the E.coli Expression System.
Purification:This protein is purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.
Approx. Molecular Weight:84kD.
Specificity: This protein shows very high binding affinity to T.cruzi (Chagas) converted human serum polyclonal antibodies in ELISA and Western Elisa. Qualified for one-step immunochromatographic diagnostic tests.
Biological Activity: Not done
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml, 1ul/cm.