PRODUCT# 1052: Recombinant tat HIV-1 Bal
Concentration: 1mg/ml ELISA
Mass/vial: 100ug Western ELISA
Volume/vial: See vial Anti-tat Drug Screening
Diluent: 50mM Tris, 0.1M NaCl, 2mMdtt, pH7.8 Immunization
Purity : >95% Transcriptional Activation
Physical State: Frozen Liquid
Stability: At least 6 months at -75oC.
DESCRIPTION: Full length (101 amino acids) Recombinant HIV-1 Bal tat produced in the E. coli Expression System.
PURIFICATION: This protein was purified by solvent extraction, ion affinity and macro-molecular sieving to approx.95% purity, as determined by SDS-PAGE , reduced. MW: approx. 16kD.
SPECIFICITY: This protein binds to murine monoclonal antibodies of defined epitope specificity and rabbit and human serum polyclonal antibodies in ELISA and Western ELISA.
BIOLOGICAL ACTIVITY: Not Done.
ENDOTOXIN: Assay Pending.
APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute tat stock solution in Tris- saline or salins-phosphate buffer, pH 7-8, immediately before use. Tat readily oxidizes in buffer solutions which may change its LTR- dependent transcriptional activation activity.
Transcriptional activation assays with tat are performed in 1-10µg/ml range. ELISA and Western ELISA require tat in 10-100ng protein range.