Product# 1121: Murine anti-gp120 HIV-1 Monoclonal Antibody
Antibody Class: IgG2b ELISA, Western ELISA
Concentration: See vial Immunoprecipitation
Mass/vial: 100µg Immunocytochemistry
Purity: >95% Immunohistochemistry
Stabilizer: None In Vitro Virus Neutralization
Physical State: Frozen Liquid
Stability: At least 12 months at -75oC
DESCRIPTION: High affinity murine anti-gp120 HIV-1 mAb. Epitope: 315 to 330 amino acids of HIV-1 gp120.
SPECIFICITY: Binds to V3-loop of HIV-1 gp120/gp160. Cross reactive with MN, IIIB, RF, SF2 and other primary HIV-1 isolates.
BIOLOGICAL ACTIVITY: Neutralizes different strains of HIV-1 at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 infected cells and co-cultures of HIV-1 infected human PBLs.
APPLICATIONS AND INSTRUCTIONS FOR USE
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays. Western ELISA require at 1-5µg/ml mAb..