Product Specifications:
Item # 1301-F: Fluorescene Rabbit Anti-gp120 HIV-1 IIIB pAb IgG
Mass/vial: Approx. 50ug IgG
Vol/vial: 100ul
Diluent: 10mM Tris, pH 8.0
Stabilizer: None
Preservative: None
Storage: -75°C
F/P Ratio: 2 - 4
Physical State: Frozen Liquid
Stability: At least 12 months at -75°C
Minimum Dilution: 1 : 50
Applications: Immunohistocytology, Immunostaining/sorting, Dot Blots, FACS.
Description: Fluorescene conjugated Rabbit anti-gp120 (HIV-1 IIIB) pAb IgG.
Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This fluorescene conjugated pAb IgG binds to native and recombinant HIV-1 gp120 in Dot Blot assays and stains cell surfaces in direct immunofluorescence assays.
Biological Activity: None
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified gp120 may be performed at a minimum dilution of 1 : 100.
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