Item # 1107-F: Fluorescene Murine Anti-vif HIV-1 mAb IgG
Mass/vial: Approximately 50µg IgG
Diluent: 10mM Tris, pH 8.0
F/P Ratio: 3 - 4
Physical State: Frozen Liquid
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 50
Applications: Dot Blots, Immunohistocytology.
Description: Fluorescene conjugated Murine Anti-vif HIV-1 mAb.
Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.
Specificity: This fluorescene conjugated mAb binds to native and recombinant HIV-1 vif as determined by dot blot and Western blot assays.
Biological Activity: None
Application and Instruction for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dot Blot assays with purified vif may be performed in 1 : 200 dilution range. vif may be detected in viral lysates by direct fluorescent assays.