Item # 2503: Human Anti-p24 HIV-1 IIIB pAb IgG
Concentration: See vial
Physical State: Frozen Liquid
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 1000
Application: ELISA/p24 Western ELISA, Immunoprecipitation, Immunohistochemistry, Virus Neutralization Assays, FACS, p24 Capture ELISA.
Description: pAb IgG fraction purified from HIV-1 immune human serum .
Purification: This pAb IgG was purified by rp24 affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This pAb IgG binds to native and recombinant HIV-1 p24 in ELISA and Western ELISA.
Biological Activity: None
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. This pAb IgG yields titres of greater than 1 : 2000 with 100ng of immobilized p24 (IDI Product# 1003) under standard conditions. ELISA and Western ELISA may be performed at 1-2µg/ml of pAb depending on the nature and the quantity of the immobilized antigen. Histochemical studies require dilutions in 1 : 200 dilution range.
Caution: Avoid the use of needles and ensure proper laboratory safety precautions are in place for the use of this product.