Peroxidase Murine mAb gp120 HIV-1

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ImmunoDXSKU: 1101-P

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PRODUCT# 1101-P:  Peroxidase-Conjugated Murine Anti-gp120 IIIB mAb


SPECIFICATIONS                                                                    APPLICATIONS

  Mass/vial Approx. 25µg                                                       ELISA/Western ELISA

  Volume/vial 100µl                                                                Histocytochemistry

  Diluent 10mM Tris, pH 8                                                     Human T-cell Research

   1% BSA                                                                                Drug Screening

  Preservative: 0.01% Thimerasol                                         gp120 Capture Assays

  Storage: 4oC

  Stability: At least 3 months at 4oC

  Minimum Dilution: 1 : 1000

  

DESCRIPTION: Peroxidase conjugated Murine anti-gp120 IIIB mAb.


MOLAR RATIO: 2 - 4 peroxidase molecules per molecule of mAb.


SPECIFICITY: Peroxidase conjugated Murine anti-gp120 mAb binds to native and recombinant gp120 IIIB (V3 loop specific) in ELISA and Western ELISA.

BIOLOGICAL

ACTIVITY: Peroxidase conjugated Murine anti-gp120 mAb binds to gp120 in CD4/gp120 capture ELISA and Western ELISA.


APPLICATIONS AND INSTRUCTIONS FOR USE


Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.


  1. ELISA and Western ELISA may be performed at dilutions of 1 : 5000 depending on the concentration of gp120 in the test system.  CD4/gp120 capture ELISA may be performed in 1 : 500 dilution range depending on the capture ligand CD4 concentration and gp120 concentration in the test sample.  Typical assays with 100ng of solid phase CD4 will detect at least 10ng/ml gp120.

PRODUCT#1101: Murine anti-gp120 IIIB mAb

Application:  gp120 ELISA, CD4/gp120 capture ELISA, Drug Screening, Diagnostics.

PRODUCT#1101-G: Colloidal gold-Conjugated Murine anti-gp120 mAb

Application:  CD4 binding, Drug screening, Histocytology, CD4/gp120 Dot Blot.

PRODUCT# 1101-F: Flourescene-Conjugated Murine anti-gp120 mAb

Application:  FACS analysis, Histocytology.

PRODUCT#:  1021-M: Magnetically-Coupled Murine anti-gp120 mAb

Application:  Human diagnostics, Human B and T-cell research, Immunoprecipitation.


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