Item#1001-G: rgp120 HIV-1 IIIB Conjugated to Colloidal Gold
Concentration: See vial
Volume/vial: 1ml Gold Conjugate
Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3
Stability: At least 3 months at 4°C Minimum
Dilution: 1 : 50
Application: Dot Blot Assay-CD4 Binding, Dot Blot Assay-Anti-gp120 Antibody Binding, Immunohistochemistry.
Description: rgp120 HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size).
Coupling Ratio: OD 520nm = 10; protein content approximately 25µg.
Specificity: Gold conjugated rgp120 binds to murine monoclonal antibodies of defined epitope specificity and human polyclonal antibodies as determined by dot blot assays.
Biological Activity: Not determined.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
1. Dot blot assays may be performed in 1 : 100 dilution range depending on the concentration of solid phase CD4/Anti-gp120 in the test system.
2. Immunohistochemical studies may be performed in 1 : 50 dilution range.
Item#: 1001-P Peroxidase-Conjugated rgp120 HIV-1 IIIB
Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.
Item#: 1001-F Flourescene-Conjugated rgp120 HIV-1 IIIB
Application: FACS analysis, Histocytology.
Item#: 1001-M Magnetically-Coupled rgp120 HIV-1 IIIB
Application: Human diagnostics, Human B and T-cell panning